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Butterfly differential deuterium uptake plot

plot_differential_butterfly.Rd

Butterfly plot of differential deuterium uptake values between two biological states in time.

Usage

plot_differential_butterfly(
  diff_uptake_dat = NULL,
  diff_p_uptake_dat = NULL,
  theoretical = FALSE,
  fractional = FALSE,
  show_houde_interval = FALSE,
  show_tstud_confidence = FALSE,
  uncertainty_type = "ribbon",
  confidence_level = 0.98,
  interactive = getOption("hadex_use_interactive_plots")
)

Arguments

diff_uptake_dat

data produced by create_diff_uptake_dataset function.

theoretical

logical, determines if values are theoretical

fractional

logical, determines if values are fractional

show_houde_interval

logical, determines if houde interval is shown.

show_tstud_confidence

logical, determines if t-Student test validity is shown.

uncertainty_type

...

confidence_level

confidence level for the test, from range [0, 1]. Important if selected show_confidence_limit.

interactive

logical, whether plot should have an interactive layer created with with ggiraph, which would add tooltips to the plot in an interactive display (HTML/Markdown documents or shiny app).

Value

a ggplot object.

Details

Function plot_differential_butterfly generates differential butterfly plot based on provided data and parameters. On X-axis there is peptide ID. On the Y-axis there is deuterium uptake difference in chosen form. Data from multiple time points of measurement is presented. If chosen, there are confidence limits based on Houde test on provided confidence level.

References

Houde, D., Berkowitz, S.A., and Engen, J.R. (2011). The Utility of Hydrogen/Deuterium Exchange Mass Spectrometry in Biopharmaceutical Comparability Studies. J Pharm Sci 100, 2071–2086.

See also

read_hdx create_diff_uptake_dataset

Examples

diff_uptake_dat <- create_diff_uptake_dataset(alpha_dat)
plot_differential_butterfly(diff_uptake_dat = diff_uptake_dat)

plot_differential_butterfly(diff_uptake_dat = diff_uptake_dat, show_houde_interval = TRUE)


diff_p_uptake_dat <- create_p_diff_uptake_dataset(alpha_dat)
plot_differential_butterfly(diff_p_uptake_dat = diff_p_uptake_dat, show_tstud_confidence = TRUE)